Naslov
Application of Wave Bioreactor for the Production of BHK 21 and CHO Cells Biomass

Autori
Slivac, Igor ; Gaurina Srcek, Višnja ; Radosevic, Kristina ; Kniewald, Zlatko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Toxicolgy Letters, Volume 158 (2005) Suppl. 1, Abstracts of the 42nd Congress of the European Societies of Toxicology, EUROTOX 2005 Cracow, Poland, September 11th-14th, 2005 / Kehrer, J.P. ; Dekant, W. ; Smith, C. - Amsterdam : Elsevier, 2005

Skup
EUROTOX 2005 - the 42nd Congress of the European Societies of Toxicology

Mjesto i datum
Kraków, Poljska, 11.09.2005. - 14.09.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
BHK 21 C13 cells; CHO K1 cells; biomass; micocarriers; Wave bioreactor

Sažetak
Stable bioprocess systems are a demand for cultivation and maintaining of various cell lines used for pharmaceutical, toxicological or biomedical applications. The Wave bioreactor 20 SPS as a novel bioprocess system was used for biomass production of CHO-K1 and BHK 21 C13 cells. During the period of 62 days a repeated fed-batch cultivation of suspended BHK 21 C13 cells was performed. Maximum cell concentration of 5, 2 x 10^6 was achieved with viability of more than 90%. The viability of the cells was determined using trypan blue exclusion method. The scale-up of 1:10x of that system provide a significant amount of cells. The CHO-K1 cells were attached on Cytoline 1 microcarriers, which have macroporous structure suitable for cultivation of adherent cells. We monitored their growth on the microcarriers in order to determine time needed for cells to overgrow them. The number of cells for total overgrowing is approx. 8.000/microcarrier. Still, limited data on viability, and quantification of cells attached to the Cytoline 1 are available Cell harvesting with 0, 25 % trypsin shows just an outer layer of cells due to macroporous structure of the microcarriers. In order to avoid this limitation we performed standard MTT viability assay to determine number of viable cells grown on the microcarriers. The obtained BHK21 C13 cells and CHO-K1 cells were later used for toxicological investigations and were proved as a standard quality. This system provides possibility for the production of other cells faster and cheaper than with previous systems.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA