Naslov
ASSESSMENT OF THE RADIOPROTECTIVE EFFECTS OF AMIFOSTINE AND MELATONIN ON HUMAN LYMPHOCYTES IRRADIATED WITH GAMMA RAYS IN VITRO

Autori
Kopjar, Nevenka ; Miočić, Slavica ; Ramić, Snježana ; Milić, Mirta ; Viculin, Tomislav

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Zbornik radova Šestog simpozija Hrvatskog društva za zaštitu od zračenja / Garaj-Vrhovac, Verica ; Kopjar, Nevenka ; Miljanić, Saveta - Zagreb : Hrvatsko društvo za zaštitu od zračenja, 2005, 194-199

Skup
VI. Simpozij Hrvatskog društva za zaštitu od zračenja s međunarodnim sudjelovanjem

Mjesto i datum
Stubičke Toplice, Hrvatska, 18.04.2005. - 20.04.2005

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
amifostine; melatonin; radioprotective effects

Sažetak
Radioprotective effects of amifostine and melatonin on human peripheral blood irradiated with gamma-rays were investigated using the micronucleus (MN) assay and the analysis of sister chromatid exchanges (SCE). Duplicate blood samples were pre-treated with amifostine (7.7 mM), melatonin (2 mM) and their combination for 30 minutes. Negative controls were also included. After treatment with radioprotectors, one blood sample from each experimental group was exposed to gamma-rays from a 60Co source. The radiation dose absorbed was 2 Gy. Pre-treated irradiated blood samples showed a decrease in the total number of MN and in the number of cells with more than one MN. Moreover, they also showed significantly lower mean SCE values. Our results indicate that amifostine, melatonin and their combination in vitro have radioprotective effects on gamma-irradiated human peripheral blood lymphocytes, with no significant genotoxicity. Therefore, it may be reasonable to use them in combination, adjusting the doses of amifostine to achieve the best radioprotective effect with as few side effects as possible. Before employment, this combination should be extensively tested in vitro and in vivo, using the same and other biomarkers for different radiation dose and concentration ranges of both radioprotectors.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA