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Pregled bibliografske jedinice broj: 205079

Influence of p53 status on the HSV-Tk/GCV-induced bystander effect in a panel of human ovarian carcinoma cell lines

van Dillen, I.J.; Mulder, N.H.; Sluiter, W.J.; Meijer, C.; de Jong, S.; Lončarek, Jadranka; Mesnil, M.; de Vries, E.F.; Vaalburg, W.; Hospers, G.A.
Influence of p53 status on the HSV-Tk/GCV-induced bystander effect in a panel of human ovarian carcinoma cell lines // Oncology research, 15 (2005), 3; 151-159 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 205079 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Influence of p53 status on the HSV-Tk/GCV-induced bystander effect in a panel of human ovarian carcinoma cell lines

Autori
Van Dillen, I.J. ; Mulder, N.H. ; Sluiter, W.J. ; Meijer, C. ; de Jong, S. ; Lončarek, Jadranka ; Mesnil, M. ; de Vries, E.F. ; Vaalburg, W. ; Hospers, G.A.

Izvornik
Oncology research (0965-0407) 15 (2005), 3; 151-159

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
gap junction; intercellular communication; p53; HSV-Tk/GCV

Sažetak
The p53 protein is mutated in 50% of all human tumors and plays a key role in apoptosis, cell cycle, and the expression of several genes. We investigated if p53 mutations influence the herpes simplex virus thymidine kinase (HSV-tk)/ganciclovir (GCV)-induced bystander effect (BE). Additionally, we studied some of the underlying mechanisms. GCV sensitivity and BE were studied in a human ovarian carcinoma cell line transfected with an empty vector (A2780/cmv) or a vector containing a p53 hotspot mutation at codon 175 (A2780/m175) or 248 (A2780/m248). In addition, expression levels of two nucleoside analogue transporters, multidrug resistance-related protein 4 and 5 (MRP4/MRP5), were determined. Finally, differences in gap junctional intercellular communication (GJIC) were studied by determining connexin 43 (Cx43) expression and by modulating GJIC with 18-alpha-glycyrrhetinic acid. Our results showed that compared to A2780/cmv, GCV sensitivity was significantly decreased in A2780/m175 and A2780/m248. Additionally, a significant BE (relative increase in cell kill) was found in A2780/cmv and A2780/m248. In contrast, an increased survival was observed in A2780/m175. No MRP4 or MRP5 expression was detected. However, all A2780 cell lines expressed Cx43. Modulating the GJIC significantly decreased the BE in A2780/cmv and A2780/ m248. In conclusion, HSV-tk/GCV-induced BE is influenced by p53 mutations. Differences in GJIC could be one of the underlying mechanisms.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb

Profili:

Avatar Url Jadranka Lončarek (autor)

Citiraj ovu publikaciju:

van Dillen, I.J.; Mulder, N.H.; Sluiter, W.J.; Meijer, C.; de Jong, S.; Lončarek, Jadranka; Mesnil, M.; de Vries, E.F.; Vaalburg, W.; Hospers, G.A.
Influence of p53 status on the HSV-Tk/GCV-induced bystander effect in a panel of human ovarian carcinoma cell lines // Oncology research, 15 (2005), 3; 151-159 (međunarodna recenzija, članak, znanstveni)
Van Dillen, I., Mulder, N., Sluiter, W., Meijer, C., de Jong, S., Lončarek, J., Mesnil, M., de Vries, E., Vaalburg, W. & Hospers, G. (2005) Influence of p53 status on the HSV-Tk/GCV-induced bystander effect in a panel of human ovarian carcinoma cell lines. Oncology research, 15 (3), 151-159.
@article{article, author = {van Dillen, I.J. and Mulder, N.H. and Sluiter, W.J. and Meijer, C. and de Jong, S. and Lon\v{c}arek, Jadranka and Mesnil, M. and de Vries, E.F. and Vaalburg, W. and Hospers, G.A.}, year = {2005}, pages = {151-159}, keywords = {gap junction, intercellular communication, p53, HSV-Tk/GCV}, journal = {Oncology research}, volume = {15}, number = {3}, issn = {0965-0407}, title = {Influence of p53 status on the HSV-Tk/GCV-induced bystander effect in a panel of human ovarian carcinoma cell lines}, keyword = {gap junction, intercellular communication, p53, HSV-Tk/GCV} }
@article{article, author = {van Dillen, I.J. and Mulder, N.H. and Sluiter, W.J. and Meijer, C. and de Jong, S. and Lon\v{c}arek, Jadranka and Mesnil, M. and de Vries, E.F. and Vaalburg, W. and Hospers, G.A.}, year = {2005}, pages = {151-159}, keywords = {gap junction, intercellular communication, p53, HSV-Tk/GCV}, journal = {Oncology research}, volume = {15}, number = {3}, issn = {0965-0407}, title = {Influence of p53 status on the HSV-Tk/GCV-induced bystander effect in a panel of human ovarian carcinoma cell lines}, keyword = {gap junction, intercellular communication, p53, HSV-Tk/GCV} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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