Naslov
Differencies in genotoxicity of atrazine as pure active ingredient and atrazine based pesticide formulation

Autori
Želježić , Davor ; Garaj-Vrhovac , Vera

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the 2nd Congress of Croatian Geneticists / Franekić-Čolić , Jasna ; Ugarković , Đurđica - Zagreb, 2005

Skup
2nd Congress of Croatian Geneticists

Mjesto i datum
Supetar, Hrvatska, 24.09.2005. - 27.09.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
atrazin; formulacija pesticida; genotoksičnost; kometni test
(atrazine; pesticide formulation; genotoxicity; comet assay)

Sažetak
Atrazine was introduced at the market in 1958 as a triazine herbicide used for the control of broadleaf weeds. It is mostly applied pre-emergence to corn, sorghum, coffee, wheat, sugarcane, in conifer forests, on Christmas tree farms on golf courses and residential lawns. Until now there are more than 100 different pesticide formulations with atrazine as active ingredient. Beside atrazine they contain various chemical compounds that should facilitate pesticide application, atrazine penetration into the plant and increase its toxicity. In this work human lymphocytes were treated with three different concentrations (0, 047 μ g/ml, 0, 47 μ g/ml, 4, 7 μ g/ml) of both, atrazine and Gesaprim&reg ; , its formulation. Lymphocytes were treated for 30 minutes and 8 hours. We measured DNA damaging potency of atrazine and Gesaprim&reg ; by alkaline comet assay. For atrazine as pure active ingredient we observed significant increase in DNA migration ability only at the highest concentration used after 8 hours of treatment. At all three concentrations tested Gesaprim&reg ; significantly increased the tail length and tail intensity values even after the 30 minutes of treatment. According to these results it is evident that genetoxicity of pure active ingredient and its commercial formulation differ significantly. It could be due to genotoxic effect of additives in the formulation themselves and/or their effect on elevating of the atrazine genotoxicity potential.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA