Naslov
Determination of micronuclei in V79 cells induced by cadmium chloride using giemsa, dapi and silver staining techniques

Autori
Milić, Mirta ; Rozgaj, Ružica ; Kašuba, Vilena

Izvornik
Toxicology letters (0378-4274) 158 (2005), Suppl 1;

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kongresno priopcenje, znanstveni

Ključne riječi
cadmium chloride; V79 cells; cytochalasin B-blocked micronucleus test; Giemsa; DAPI and Silver staining

Sažetak
Soluble compounds of highly toxic heavy metals are well known as inductors of genotoxic damages in humans and animals. We evaluated the toxic effects of cadmium chloride as one of them. The induced DNA damage was observed through the formation of micronuclei (MN) in Chinese hamster cells. V 79 cells were simultaneusly treated with different doses of cadmium chloride (10-4, 10-5, 10-6 M) for 24 h. MMC- treated cells served as positive control. Cytochalasin-B was added to arrest cytokinesis.Three separate cultures per concentration were analyzed. Slides were stained using conventional Giemsa, DAPI (4´, 6-diamidino-2-phenylindole-dihydrochloride) and silver staining techniques. For each staining technique, 1000 binucleated cells per slide were analysed. All techniques revealed an increased number of MN when compared to control. DAPI staining gave more information about the origin of MN and allowed us to distinguish between MN+ and MN-. In respect to the paterns of nucleolar organizer (NOR) activity in MN we distinguish Ag-NOR+ and Ag-NOR- MN.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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