Naslov
Evidence for apparent gene instability in the rifamycin producing polyketide synthase: implications for combinatorial biosynthesis and heterologous gene expression

Autori
Perić-Concha, Nataša ; Castaldo, Gaetano ; Long, F. Paul

Izvornik
Folia microbiologica (0015-5632) 50 (2005), 6; 483-486

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
AMYCOLATOPSIS MEDITERRANEI S699; ESCHERICHIA-COLI; B BIOSYNTHESIS; POLYKETIDE; CLUSTER; CLONING

Sažetak
The oligoketide ('polyketide') synthase leading to the formation of proansamycin X in Amycolatopsis mediterranei also prematurely releases a range of acyclic intermediates from the enzyme complex. We intended to study the chemical biology of this ectopic chain release using RifA as a model protein system ; however, we were unable to clone the rifA gene in its entirety. Restriction analysis of cosmid clones revealed that rifA is subject to random deletions at high frequency, especially in central regions of the locus. Examination of the gene sequence in this region reveals a high concentration of inverted repeats ; we suggest that these sequences are subject to alteration in secondary structure when cloned outside the environment of the A. mediterranei genome, leading to recombination and deletion.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA