Naslov
TLC analysis of free amino acids in Valeriana officinalis

Autori
Gašpar Randić, Zita ; Maleš, Željan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Final Program and Book of Abstracts 53 rd Annual Congress of the Society for Medicinal Plant Research / F.F. Vincieri - Firenza : München : Societa italiana di fitochimica, 2005

Skup
53rd Annual Congress of the Society for Medicinal Plant Research

Mjesto i datum
Firenca, Italija, 21.08.2005. - 25.08.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Valeriana officinalis L.; Valerianaceae; amino acids; TLC

Sažetak
Valeriana officinalis L. is a perennial herbaceous plant, belonging to the Valerianaceae family. The drug Valerianae radix consists of subterranean parts of the plant including the rhizomes, roots and stolons, carefully dried below 40 0C. It contains volatile oil, iridoids, flavonoids, triterpenes, alkaloids, amino acids, salts, sugars, and organic acids. Because there are only few data about free amino acids in this plant, a TLC as a rapid method was developed for its investigation. Leaves, stems, flowers and roots of V. officinalis were collected in the Pharmaceutical Botanical Garden “ Fran Kušan” in June and October 2004. These plant parts were investigated in comparison with Valerianae radix from commercial origin. TLC method has been performed on cellulose and silica gel, using n-butanol-acetone-glacial acetic acid-water (35:35:10:20 V/V/V/V) as solvent system. Detection has been carried out by a ninhydrin reagent. The TLC method proved that the amino acid composition depended on the plant part and its origin. Leaves and flowers collected in June contained more amino acids (alanine, phenylalanine, valine, tryptophan and tyrosine) in comparison with stalk and root. The root collected in October was richer in amino acids (glutamine, arginine, alanine and  -aminobutyric acid) than the root collected in June, because in October the root became the deposit organ, in which the reserves of glutamine and arginine formed. The sample of root from commercial origin contained glutamine, arginine, alanine and  -aminobutyric acid.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

POVEZANOST RADA