Naslov
Spinsko obilježavanje ovalbumina
(Spin labelling of ovalbumin)

Autori
Brgles, Marija ; Mirosavljević, Krunoslav ; Frkanec, Ruža ; Noethig Laslo, Vesna ; Tomašić, Jelka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XIX. hrvatski skup kemičara i kemijskih inženjera: knjiga sažetaka / Rapić, Vladimir ; Rogošić, Marko - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI) ; Hrvatsko kemijsko drustvo, 2005, 90-90

ISBN
953-6894-23-8

Skup
Hrvatski skup kemičara i kemijskih inženjera (19 ; 2005)

Mjesto i datum
Opatija, Hrvatska, 24.04.2005. - 27.04.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
spinsko označavanje; ovalbumin; elektronska spinska rezonancija
(spin labelling; ovalbumin; electron spin resonance)

Sažetak
Ovalbumin (OVA) is the major protein in avian egg-white and was one of the first proteins to be isolated in a pure form. It belongs to the serpin (serine protease inhibitors) family but it lacks any protease inhibitory activity. Its ready availability in large quantities has led to its wide- spread use as a standard preparation in studies of the structure and properties of proteins, and in experimental models of allergy [1]. Among the others OVA has been used as a model protein antigen in our investigations of adjuvant effects of immunostimulating peptides [2]. Spin labelling was succesfully applied in the study of interactions of peptidoglycan monomer and structurally related adamantyltripeptides with lipid bilayers in liposomes [3]. The aim of the present study has been to investigate the possible interactions of OVA with immunostimulating peptides and with liposomal bilayers by electron spin resonance (ESR). Ovalbumin was spin labelled with 4-maleimido-2, 2, 6, 6, -tetramethylpiperidine-1-oxyl (TEMPO-maleimide). Spin labelled OVA (SL-OVA) was purified by column chromatography. Fractions containing SL-OVA were concentrated by ultrafiltration on Centricon Centrifugal Filter Devices. The interactions of SL-OVA with phospholipids in liposomal bilayers and with immunological adjuvant peptidoglycan monomer were investigated. The ESR study demonstrated that there was no interaction between OVA as an antigen and peptidoglycan monomer as adjuvant. Also no interactions with liposomal membranes were detected. On the other hand, the reaction of OVA as an antigen and OVA-specific antibodies was recorded as a change in EPR spectra of SL-OVA. Thus it was also demonstrated that spin labelling has no influence on binding of the antibodies to OVA. [1] J.A. Huntington, P.E. Stein, J. Chromatogr. B. 756 (2001) 189-198. [2] J. Tomašić, I. Hanzl-Dujmović, B. Špoljar, B. Vranešić, M. Šantak, A. Jovičić, Vaccine 18 (2000) 1236-1243. [3] R. Frkanec, V. Noethig Laslo, B. Vranešić, K. Mirosavljević and J. Tomašić, Biochim. Biophys. Acta, 1611 (2003) 187-195.

Izvorni jezik
Hrvatski

Znanstvena područja
Kemija, Temeljne medicinske znanosti

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