Naslov
Autocrine tumor growth regulation by somatomedin C : an in vitro model

Autori
Pavelić, Krešimir ; Vrbanec, Damir ; Marušić, Suzana ; Levanat, Sonja ; Čabrijan, Tomislav

Izvornik
Journal of endocrinology (0022-0795) 109 (1986); 233-238

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
autocrine growth factor ; somatomedin C ; in vitro model

Sažetak
A human primary hemangiosarcoma was derived from a patient with severe hypoglicemia. Cell line established from that tumor secreted somatomedin C in serum-free culture media. Immunoreactive somatomedin from that media eluted from Sephacryl S-200 in two peaks of 160 000 and 8 000 molecular weights. Similar results were obtained when medium was acidified and chromatographed on Sephadex G- 50. Binding of tracer concentrations of 125J- labelled somatomedin C to human haemangiosarcoma cells was much higher than that of 125J-labelled insulin. Half maximal displacement of 125J- labelled somatomedin C binding occurred at an unlabelled somatomedin C concentration of 0.7 nmol/l. Insulin competed with 125J-labelled somatomedin for binding to this receptor, but 150- fold more insulin was required for half-maximal displacement. Somatomedin secreted by human haemangiosarcoma cells and purified from serum- free media strongly stimulated /methyl- 3H/thymidineincorporation into the DNA of these cells. Inhibition of somatomedin C secreted by cortisol resulted in the inhibition of tumor cell proliferation but stimulation of somatomedin secretion by human GH stimulated the cell proliferation rate. It appears that production of somatomedin C in human haemangiosarcoma cellsplays a part in the regulation of tumour growth by an autocrine mechanism.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA