Naslov
Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92

Autori
Kos, Blaženka ; Šušković, Jagoda ; Vuković, Snježana ; Šimpraga, Miljenko ; Frece, Jadranka ; Matošić, Srećko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Prvi kongres hrvatskih znanstvenika iz domovine i inozemstva - Zbornik sažetaka postera znanstvenih novaka izlaganih u inozemstvu 2002., 2003. i 2004. godine, I dio Prirodne, tehničke i biotehničke znanosti / Kniewald, Zlatko - Zagreb : Akademija tehničkih znanosti Hrvatske (HATZ), 2004, 445-445

Skup
Prvi kongres hrvatskih znanstvenika iz domovine i inozemstva

Mjesto i datum
Vukovar, Hrvatska; Zagreb, Hrvatska, 15.11.2004. - 19.11.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
adhesion; aggregation; S-layer; Lactobacillus acidophilus; probiotic properties

Sažetak
The ability of probiotic bacteria to adhere to the intestinal epithelium play an important role in colonization of the gastrointestinal tract, preventing their elimination by peristalsis and providing a competitive advantage in this ecosystem. To identify bacterial traits related to adhesion the probiotic strain L. acidophilus M92 was examined for autoaggregation ability and cell surface hydrophobicity L. acidophilus M92 exhibits a strong autoaggregation phenotype and also coaggregation with some pathogen microorganisms that may form a barrier that prevents their colonization. The examined probiotic strain manifests a good degree of hydrophobicity determined by microbial adhesion to hydrocarbons. Aggregation and hydrophobicity were abolished upon exposure of the cells to pronase, which suggests the possible role of cell surface layer (S-layer) proteins, approximated at 45 kDa, in a L. acidophilus M92. The relationship between autoaggregation and adhesion ability to intestinal tissue was investigated by observing the adhesivity of L. acidophilus M92 to porcine ileal epithelial cells. Removal of the S-layer proteins by extraction with 5 M LiCl reduced autaggregation and in vitro adhesion of this strain.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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