Naslov
DNA Microarray analysis of gene expression induced by ovalbumin challenge in Balb/c mice asthma model

Autori
Bošnjak, Berislav ; Ivetić, Vanesa ; Hrvačić, Boška

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts / Dumić, Jerka - Zagreb : Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 2004, 63-63

Skup
Congress of the Croatian society of biochemistry and molecular biology

Mjesto i datum
HOC Bjelolasica, Hrvatska, 30.09.2004. - 02.10.2004

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
DNA; microarray; asthma; gene expression; mouse; lung

Sažetak
Asthma is chronic inflammatory disorder of the airways with recurrent exacerbations. Despite intensive ongoing research, cellular and molecular events that led to the development and progression of asthma are still not completely understood. However, in recent years DNA microarray technique made possible expression analysis of thousands of genes simultaneously and provided genome wide insight into (patho)physiological processes in the cell, tissue or organ of interest. Study aimed to identify, by DNA microarray technique, set of genes whose expression is changed at the beginning of asthmatic attack. Gene expression in lungs of Balb/c male mice previously sensitized to ovalbumine (OVA) and 4 hours after intranasal challenge with OVA was compared to expression in lungs from mice challenged with PBS. RNA from lungs was isolated using TRIZOL Reagen (Gibco). RNA quality was checked on Bioanalyzer (Agilent) and RNA amplification, labeling and hybridization on DNA microarray slides (MO3, Mergen) was performed according to manufacturer recommendations. Slides were scanned on ScanArray Lite scanner (Perkin Elmer Life Sciences) and obtained pictures were analyzed using ExpressWare (Mergen). Data analysis was performed using GeneSifter.Net (Vizx Labs). Among investigated 8550 genes, challenge 352 (~4.1%) had significantly different expression: 203 up-regulated and 149 down-regulated. Genes whose expression was changed had widely distributed functions, but preferentially included genes involved in immunity. Further, 12 differently expressed genes have still unknown function (RIKEN expressed sequences). DNA microarray analysis of gene expression in mice lungs after OVA challenge revealed insight into the complex pathways involved in the asthma development. Analysis indicated that a vast number of gene products contribute to the complexity of asthma pathogenesis. Functional classification of genes which expression was changed identified diverse processes besides immunity, including development, signal transduction and intracellular transport. Some of these genes could become novel gene-targets for development of asthma treatment.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA