Naslov
Histological assessment of wound healing dynamics in db/db diabetic mice treated with PL 14736

Autori
Čužić, Snježana ; Ivetić, Vanesa ; Brajša, Karmen ; Parnham, Michael J.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Knjiga sažetaka 3. hrvatskog kongresa patologije i sudske medicine, 3. hrvatskog kongresa kliničke citologije i 1. hrvatskog simpozija citotehnologije s međunarodnim sudjelovanjem / Jonjić, Nives ; Kardum-Skelin, Ika - Karlovac, 2005, 96-96

Skup
3. Hrvatski kongres patologije i sudske medicine, 3. Hrvatski kongres kliničke citologije, 1. Hrvatski simpozij citotehnologije s međunarodnim sudjelovanjem

Mjesto i datum
Opatija, Hrvatska, 08.05.2005. - 11.05.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
wound; healing; db/db; PL14736; re-epithelialization; granulation; PDGF-BB

Sažetak
PL 14736 is a peptide under investigation for use in wound healing. We have studied he efficacy of PL 14736, applied daily directly to excisional wounds in genetically obese, insulin resistant (db/db) diabetic mice, which exhibit delayed wound healing. The test substance was compared to the reference substance platelet derived growth factor (PDGF-BB). Female db/db mice each received an 8mm punch biopsy skin wound on the back under anaesthesia. Animals were divided into 3 groups of 25 animals each, treated daily with PDGF-BB, PL 14736 or vehicle (phosphate buffered saline) applied directly to the wound. Five animals per group were each killed on days 6, 9, 12, 18 and 24 post-wounding. Tissue was formalin-fixed, cut through the centre of the wound and embedded in paraffin “ on a side” . Slides were stained with hematoxylin-eosin or van Gieson stain for collagen and elastic fibres. Morphometric evaluation of re-epithelialization was performed together with semi-quantitative granulation tissue analysis. In db/db mice treated with PL 14736, re-epithelialization rate was higher on day 6 after wounding than in wounds treated with vehicle or PDGF-BB (66% versus 44% ; not statistically significant). Maturation of granulation tissue on day 12 post-wounding - seen as formation of collagen bundles - was significantly greater in animals treated with PL 14736 than in those treated with vehicle (p=0.048 in Mann-Whitney U test). PDGF-BB had little effect. We conclude that local treatment with PL 14736 enhances granulation tissue maturation in the first 12 days post-wounding in diabetic mice, while in agreement with the literature, PDGF-BB has a negligible effect on wound healing dynamics in this model.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA