Naslov
Expression of neutral glycosphingolipids in cytokine-stimulated human endothelial cells

Autori
Zemunik, Tatijana ; Markotić, Anita ; Marušić, Ana

Izvornik
Biochemistry Moscow (0006-2979) 69 (2004), 5; 513-519

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
endothelium; glycosphingolipids; E-selectin; TNF-alpha; immunohistochemistry

Sažetak
We compared immunohistochemical distribution of glycosphingolipids globotriosylceramide (GbOse(3)Cer) and globotetraosylceramide (GbOse(4)Cer) with that of E-selectin on human umbilical vein endothelial cells (HUVEC) stimulated with tumor necrosis factor (TNF)-alpha. HUVECs activated by TNF-alpha were characterized by the highest expression of E-selectin and greatest adhesion of HL-60 cells as well compared to stimulation with interleukin-1beta or lipopolysaccharide. HUVECs activated by TNF-alpha also stained intensely with globoside antibodies, especially with the GbOse(3)Cer-directed one, staining being redistributed in a concentration-dependent manner. These results indicate the possible role of GbOse(3)Cer and GbOse(4)Cer in immune effector mechanisms of endothelium such as adhesion.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne medicinske znanosti

POVEZANOST RADA