Pregled bibliografske jedinice broj: 193972
A Fast Immunoradiometric Assay of Human Growth Hormone
A Fast Immunoradiometric Assay of Human Growth Hormone // Food technology and biotechnology, 35 (1997), 1; 33-37 (podatak o recenziji nije dostupan, scientific note, znanstveni)
CROSBI ID: 193972 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
A Fast Immunoradiometric Assay of Human Growth Hormone
Autori
Mildner, Boris ; Gnjidić, Živko
Izvornik
Food technology and biotechnology (1330-9862) 35
(1997), 1;
33-37
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, scientific note, znanstveni
Ključne riječi
human growth hormone; acromegaly; pituitary adenoma; RIA; IRMA
Sažetak
Using the components of "Pharmacia" two-site immunoradiometric assay (IRMA) it is possible to determine human growth hormone (hGH) in serum in the time interval od approximately 2 h if the reaction is performed at 37 oC (modified IRMA). The standard IRMA, that is the assay performed at room temperature as recommended by the manufaturer last at least 4 h. Comparison of the results obtained by the standard "Pharmacia" protocol, i.e. modified IRMA vs. standard IRMA, yielded a correlation coefficient of r=0.9996. The results of hGH determinations obtained by two IRMA assays were also compared with the results obtained by standard competitive radioimmunoassay (RIA) of hGH. The following correlation coefficients were obtained: RIA vs. standard IRMA r=0.9997 ; RIA vs. modified IRMA r=0.9989. The modified IRMA procedure has found its value in determination of hGH in serum during and immediately after selective removal of the pituitary (somatotroph) adenoma.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Biologija, Kliničke medicinske znanosti
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
Uključenost u ostale bibliografske baze podataka::
- Biological Abstracts
- Chemical Abstracts
