Naslov
Neutral and polar lipid fractions fatty acid compositions of Diplodus vulgaris liver and muscle tissue

Autori
Varljen, Jadranka ; Batičić, Lara ; Sinčić-Modrić, Gordana ; Varljen, Neven ; Kapović, Miljenko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XIX. hrvatski skup kemičara i kemijskih inženjera : knjiga sažetaka / Rapić, Vladimir ; Rogošić, Marko - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa ; Hrvatsko društvo Kemija u industriji, 2005, 269-269

ISBN
953-6894-23-8

Skup
XIX. hrvatski skup kemičara i kemijskih inženjera

Mjesto i datum
Opatija, Hrvatska, 24.04.2005. - 27.04.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
lipids; fatty acid composition; fish; Diplodus vulgaris

Sažetak
The liver and muscle tissue of the common two-banded seabream, Diplodus vulgaris from the North Adriatic have been analysed. Total lipids were extracted from the samples according to Folch et al. [1]. The total lipid content amounted to 1, 1 +/- 0, 3 % in the edible muscle tissue and 4, 5 +/- 1, 0 % in the liver. Neutral (triacylglycerols) and polar (phosphatidylinositol, phosphatidylserine, phosphatidylcholine, phosphatidylethanolamine) lipid fractions were separated from the total lipid extract by thin-layer chromatography [2]. The fatty acid compositions of neutral and polar lipid fractions were determined by gas chromatography of the corresponding methyl esters. There have been identified 16 different fatty acids. The major constituents of the total fatty acids in both analyzed samples were saturates: palmitic (20, 8 - 43, 4 %) and stearic acid (6, 3 - 33, 7 %) ; monoenes: oleic (10, 0 - 33, 6 %) and palmitoleic acid (2, 1 - 11, 0 %) ; polyunsaturates: arachidonic acid (1, 5 - 8, 6 %), docosatrienoic acid (0, 8 - 11, 6 %), eicosapentaenoic (1, 1 - 5, 5 %) and docosahexaenoic acid (1, 5 - 9, 9 %). Statistically significant diference in the relative ratios of the eicosapentaenoic acid was found in phosphatidylethanolamine when comparing muscle and liver tissue (p<0, 01). The relative ratio of the docosahexaenoic acid was statistically significantly higher in liver triacylglycerols (p<0, 0005) and phosphatidylcholine (p<0, 05) when comparing with the muscle tissue. It has been shown that the fish liver contains more n-3 polyunsaturated fatty acids, which are well known for their beneficial role in human health and diseases [3]. [1] J. Folch, M. Lees, G.H. Sloane-Stanley, J. Biol. Chem. 226 (1957) 497-509. [2] R.J. Hamilton, S. Hamilton, Lipid Analysis, Oxford University Press (1992) 1-147. [3] C.H. Ruxton, S.C. Reed, M.J. Simpson, K.J. Millington, J Hum Nutr Diet. 17 (2004) 449-459.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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