Naslov
Structurally related flavonoids with antioxidative properties differentially affect cell cycle progression and apoptosis of human acute leukemia
(Structurally related flavonoids with antioxidative properties differentially affect cell cycle progression and apoptosis of human acute leukemia cells)

Autori
Rusak, Gordana ; Gutzeit, Herwig O. ; Ludwig-Mueller, Jutta

Izvornik
Nutrition research (0271-5317) 25 (2005), 2; 141-153

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
flavonoids ; cell cycle ; proliferation ; apoptosis ; antioxidant activity
(flavonoids ; cell cyclle ; proliferation ; apoptosis ; antooxidant activity)

Sažetak
Flavonoids are a group of common phenolic compounds that are ubiquitous in all vascular plants and important constituents of the human diet. The possible relationship between the chemical structure of six closely related naturally occurring flavonoids and their antioxidative properties as well as their effects on proliferation, apoptosis and cycle of human acute leukemia (HL-60) cells were investigated. Quercetin, myricetin, kaempferol and isorhamnetin were found to be potent antiproliferative agents, whereas 3'-methylquercetagetin and taxifolin were ineffective indicating that C2-C3 double bond and the 6-hydroxyl group are important structural requirements for the cytostatic effects of flavonoids. The analysis by low cytometry showed that quercetin and myricetin were the most potent apoptosis-inducing agents among the flavonoids tested. Myricetin, isorhamnetin and kaempferol induced time- and concentration – dependent alterations in number of cells in G0/G1 and S phase. Only quercetin increased the number of cells in G2/M phase. Myricetin showed the strongest scavenging activity against DPPH radicals among the flavonoids tested, but it was almost ineffective in reducing ROS level in menadione stressed HL-60 cells. Scavenging activity against DPPH radicals was dependent on the number of hydroxyl groups in the ring B of flavonoids. In contrast, kaempferol was the most effective ROS scavenger in menadione stressed cells. The presence of the 6- hydroxyl group was shown to be an unfavorable structural feature of flavonoids regarding to DPPH scavenging and antioxidative effects of flavonoids. Minor chemical differences between flavonoids tested produced significant differences concerning their effects on HL-60 cell proliferation and cycle distribution, apoptosis or their antioxidant activity.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

Napomena
Časopis "Nutrition Research" dostupan je unutar baze "Science Direct"

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