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Pregled bibliografske jedinice broj: 191314

Peptidoglycan monomer - product of biotechnology with immunomodulatory properties


Halassy, Beata; Habjanec, Lidija; Vdović, Valerija; Tomašić, Jelka
Peptidoglycan monomer - product of biotechnology with immunomodulatory properties // Biotechnology and Immuno-Modulatory Drugs : Programme and Abstracts / Croatian Society of Biotechnology (ur.).
Zagreb: Medicinska naklada, 2005. str. 24-24 (pozvano predavanje, domaća recenzija, sažetak, znanstveni)


CROSBI ID: 191314 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Peptidoglycan monomer - product of biotechnology with immunomodulatory properties

Autori
Halassy, Beata ; Habjanec, Lidija ; Vdović, Valerija ; Tomašić, Jelka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Biotechnology and Immuno-Modulatory Drugs : Programme and Abstracts / Croatian Society of Biotechnology - Zagreb : Medicinska naklada, 2005, 24-24

Skup
4th Croatian Scientific Conference on Biotechnology

Mjesto i datum
Zagreb, Hrvatska, 20.02.2005. - 23.02.2005

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
peptidoglycan monomer; immunomodulator; adjuvant

Sažetak
Peptidoglycan monomer, GlcNAc-MurNAc-L-Ala-D-isoGln-mesoDpm(eNH2)-D-Ala-D-Ala, (PGM, PLIVA, Croatia) is a natural compound originating from bacterial peptidoglycan. It was produced by a simple biotechnology procedure1. Brevibacterium divaricatum was cultured in the early exponential phase of growth in the presence of penicillin, that led to the accumulation of complex, soluble peptidoglycan (PG) - containing material in the culture fluid. Digestion of PG polymer with lysozyme and subsequent purification of hydrolysis products led to the isolation of PGM. It is a non-toxic, an apyrogenic and a water-soluble compound with completely defined chemical structure. Its immunostimulatory or adjuvant properties have clearly been demonstrated in mice model2, using ovalbumin (OVA) as an antigen. It is well known that PGM is the substrate for amidase present in the mammalian blood, and that it is very fast cleaved and excreted from the organism in the form of its metabolic products - pentapeptide and dissacharide3. It's putative metabolic products lack the adjuvant properties of the parent compound4. We hypothesised that PGM's adjuvant properties could be better exploited in adjuvant formulations that would prolong its stay in the mammals. We prepared the novel formulations of PGM and oil-based Incomplete Seppic Adjuvants, ISA 720 and ISA 206, and tested their effectiveness in mice and rabbit models. We clearly demonstrated that the novel formulations induced significantly higher levels of antigen-specific IgGs that both PGM as well as ISA adjuvants separately, and pointed out to the differences between the two experimental models used. (1Keglević et al. (1979) Biochim. Biophys. Acta 585:273 ; 2Tomašić et al. (2000) Vaccine 18:1236 ; 3Valinger et al. (1982) Biochim. Biophys. Acta 701:63 ; 4Halassy et al. (2003) Vaccine 21:971)

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Kliničke medicinske znanosti, Biotehnologija



POVEZANOST RADA


Projekti:
0021002

Ustanove:
Imunološki zavod d.d.

Profili:

Avatar Url Jelka Tomašić (autor)

Avatar Url Lidija Habjanec (autor)

Avatar Url Beata Halassy (autor)

Avatar Url Valerija Vdović (autor)


Citiraj ovu publikaciju:

Halassy, Beata; Habjanec, Lidija; Vdović, Valerija; Tomašić, Jelka
Peptidoglycan monomer - product of biotechnology with immunomodulatory properties // Biotechnology and Immuno-Modulatory Drugs : Programme and Abstracts / Croatian Society of Biotechnology (ur.).
Zagreb: Medicinska naklada, 2005. str. 24-24 (pozvano predavanje, domaća recenzija, sažetak, znanstveni)
Halassy, B., Habjanec, L., Vdović, V. & Tomašić, J. (2005) Peptidoglycan monomer - product of biotechnology with immunomodulatory properties. U: Croatian Society of Biotechnology (ur.)Biotechnology and Immuno-Modulatory Drugs : Programme and Abstracts.
@article{article, author = {Halassy, Beata and Habjanec, Lidija and Vdovi\'{c}, Valerija and Toma\v{s}i\'{c}, Jelka}, year = {2005}, pages = {24-24}, keywords = {peptidoglycan monomer, immunomodulator, adjuvant}, title = {Peptidoglycan monomer - product of biotechnology with immunomodulatory properties}, keyword = {peptidoglycan monomer, immunomodulator, adjuvant}, publisher = {Medicinska naklada}, publisherplace = {Zagreb, Hrvatska} }
@article{article, author = {Halassy, Beata and Habjanec, Lidija and Vdovi\'{c}, Valerija and Toma\v{s}i\'{c}, Jelka}, year = {2005}, pages = {24-24}, keywords = {peptidoglycan monomer, immunomodulator, adjuvant}, title = {Peptidoglycan monomer - product of biotechnology with immunomodulatory properties}, keyword = {peptidoglycan monomer, immunomodulator, adjuvant}, publisher = {Medicinska naklada}, publisherplace = {Zagreb, Hrvatska} }




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