Pregled bibliografske jedinice broj: 191060
The variability of Vipera ammodytes ammodytes venoms from Croatia - biochemical properties and biological activity.
The variability of Vipera ammodytes ammodytes venoms from Croatia - biochemical properties and biological activity. // Comparative biochemistry and physiology. C. Toxicology & pharmacology, 140 (2005), 2; 257-263 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 191060 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
The variability of Vipera ammodytes ammodytes venoms from Croatia - biochemical properties and biological activity.
Autori
Lang Balija, Maja ; Vrdoljak, Anto ; Habjanec, Lidija ; Dojnović, Biserka ; Halassy Špoljar, Beata ; Vranešić, Branka ; Tomašić, Jelka
Izvornik
Comparative biochemistry and physiology. C. Toxicology & pharmacology (1532-0456) 140
(2005), 2;
257-263
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
anticomplementary activity; immunogenicity of snake venom; phospholipase A2; snake venom; Vipera ammodytes ammodytes;
Sažetak
Vipera ammodytes ammodytes venom has been used for many years in Croatia for immunization of horses and production of specific therapeutic antivenoms. The neutralizing effectiveness of antivenoms is directly dependent on the properties of the snake venom used for immunization. Therefore, appropriate characterization of the whole venom is necessary prior to use in the immunization procedure. In the course of such analyses, the variability in biochemical properties and biological activity was observed in venoms collected from snakes originating from different parts of Croatia. The venom pools also differed with respect to time of snake collection (1992-2003). Analyses of three samples of whole venom pools were carried out revealing differences in lethal activity (LD50), minimum haemorrhagic dose (MHD), minimum necrotizing dose (MND), phospholipase A2 activity and in anticomplementary activity. SDS-PAGE electrophoretic patterns were similar, but not identical, for all tested venom pools with respect to the number of protein bands detected, but intensity of particular components differed. Preliminary immunogenicity testing in terms of determination of specific antibodies revealed similar immunogenicity and high cross-reactivity for three samples tested.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
0021002
Ustanove:
Imunološki zavod d.d.
Profili:
Branka Vranešić
(autor)
Jelka Tomašić
(autor)
Lidija Habjanec
(autor)
Beata Halassy
(autor)
Anto Vrdoljak
(autor)
Maja Lang Balija
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE