Naslov
Detection and characterization of measles virus strains in cases of subacute sclerosing panencephalitis in Croatia

Autori
Forčić, Dubravko ; Baričević, Marijana ; Zgorelec, Renata ; Kružić, Vladimira ; Kaić, Bernard ; Marušić Della Marina, Branka ; Cvitanović Šojat, Ljerka ; Tešović, Goran ; Mažuran, Renata

Izvornik
Virus research (0168-1702) 99 (2004), 1; 51-56

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
SSPE; Measles genotype; Mutations

Sažetak
Two cases of subacute sclerosing panencephalitis (SSPE), diagnosed in Croatia in 2002, were investigated. The coding regions of the matrix (M), hemagglutinin (H) and nucleoprotein (N) genes of measles virus were sequenced following direct RT-PCR amplification of viral RNA extracted from brain tissue. Phylogenetic analysis of the sequences of H and N genes, showed that both strains belonged to genotype D6. No vaccine strain was detected although both patients had been previously immunized. The comparison of analyzed sequences of two SSPE causative viruses with corresponding sequences of D6 genotype and with each other revealed a number of mutations in N and H gene sequences. In comparison to the Edmonston reference strain, the M gene of the SSPE viruses showed the characteristic biased hypermutation and a premature termination codon in one of the patients.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

POVEZANOST RADA