Naslov
Effects of vanadate on glucose production in rat hepatocytes cultured in vitro

Autori
Roša, Jagoda ; Roša, Josip

Izvornik
Diabetologia Croatica (0351-0042) 33 (2004), 2; 43-45

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
insulin; glucose production; glycogen; gluconeogenesis; glycogenolysis; vanadate

Sažetak
The aim of this study was to determine effect of vanadate on glucose production in hepatocytes cultured in vitro. Hepatocytes were isolated by a collagenase perfusion technique and cultured for 24 h in M 199 serum-free medium. The glucose production was measured by incubating the cultures in glucose-free Hanks-Hepes medium with addition 10 mmol/l pyruvate. The glucose released into the medium was determined enzymatically with glucose oxidase. Glycogenolysis was determined as net decrease in glycogen content during the incubation period. Vanadate alone in a concentration of 1 mmol/l caused a significant (p<0.01) 57% increase of glucose production during first hour, but after that period glucose production complete ceased and hepatocytes started to consume glucose, showed as a steadily and slow decrease of glucose in medium. Although insulin alone did not change glucose production, vanadate in presence of insulin even stronger (80%) increase glucose production during first hour, but after that very strongly (60%) decreased glucose in medium during second hour. Results obtained showed that vanadate decreased glucose production inhibiting gluconeogenesis

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA