Naslov
Bone morphogenetic protein-7 from serum of pregnant mice is available to the fetus through placental transfer during early stages of development.

Autori
Borovečki, Fran ; Jelić, Mislav ; Grgurević, Lovorka ; Sampath, Kuber T. ; Bosukonda, D. ; Vukičević, Slobodan

Izvornik
Nephron. Experimental nephrology (1660-2129) 97 (2004), 1; 26-32

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Bone morphogenetic protein; BMP-7 null mutation mice; Renal development; Placental transfer

Sažetak
BMP-7 is an important mediator of metanephric mesenchyme differentiation during kidney development. Gene knockout studies have shown that BMP-7 null mutation mice die shortly after birth due to renal failure, although the induction of metanephric structures has initially occurred (E11-E13). MATERIALS AND METHODS: Iodinated BMP-7 was injected into the tail vein of pregnant mice and its availability to tissues and fetuses was further analyzed by tissue uptake, LM autoradiography and SDS-PAGE electrophoresis. RESULTS: Studies on the distribution of 125I-BMP-7 injected through the tail vein of pregnant mice indicated that 125I-BMP-7 passed across the placenta and localized in developing fetal organs, in particular kidneys, up to day 14 of gestation. At later stages of pregnancy 125I-BMP-7 did not pass the trophoblasts of the placental barrier and did not enter the fetal blood vessels. CONCLUSION: The analysis of the distribution of 125I-BMP-7 from pregnant mice to fetal organs, in particular the kidney, suggests a cross-over of maternal circulating BMP-7 to the fetus through the placental barrier.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA