Naslov
Regulatory T cells in children allergic to house dust mite undergoing specific immunotherapy

Autori
Ajduk, Jakov ; Aberle, Neda ; Rabatić, Sabina ; Gagro, Alenka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Annual meeting of the Croatian Immunological Society 2004

Mjesto i datum
Opatija, Hrvatska, 08.10.2004. - 10.10.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
regulatory T cells; immunotherapy; allergy; dust mite

Sažetak
Regulatory T cells (Treg) are defined by their surface phenotype (CD4+ CD25+ CD45RO+ CD69- CTLA- 4+) or by their ability to secrete immunoregulatory cytokines, such as interleukin (IL)-10 and transforming growth factor (TGF)- beta. The aim of this study was to detect Treg in peripheral blood from children allergic to Dermatophagoides pteronyssinus undergoing specific immunotherapy (n=15). Treg were identified using four - color flow cytometry prior to, after three months, and after one year of immunotherapy. In addition, intracellular detection of IL-10 and TGF-beta in T cells was performed. The level of CTLA-4 expressing Tregs increased transitory after 3 months of immunotherapy. While no changes in IL-10 secreting T cells were found, specific immunotherapy increased percentage of TGF-beta secreting T cells. These results indicate that analysis of CTLA-4 and TGF-beta by flow cytometry could be useful markers to monitor the effect of specific immunotherapy in allergic individuals.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA