Naslov
Unilateral flexibility in tRNASer recognition by heterologous seryl-tRNA synthetases

Autori
Rokov-Plavec, Jasmina ; Bilokapić, Silvija ; Gruić-Sovulj, Ita ; Močibob, Marko ; Glavan, Filip ; Brgles, Marija ; Weygand-Đurašević, Ivana

Izvornik
Periodicum biologorum (0031-5362) 106 (2004); 147-154

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
seryl-tRNA synthetase ; tRNA ; macromolecular recognition ; protein biosynthesis

Sažetak
Aminoacyl-tRNA synthetases (aaRSs) are ancient proteins that establish the rules of the genetic code through aminoacylation reactions, where a specific amino acid is joined to its cognate tRNA. It is believed that aaRSs and tRNA isoacceptors specific for a particular amino acid have coevolved and, as a consequence, heterologous recognition across taxonomic domains is often prevented. tRNAs were generated by in vitro transcription of corresponding synthetic genes, while the synthetases were isolated from bacterial or yeast overproducing strains. The macromolecular interactions were studied by aminoacylation and gel-shift electrophoresis, under equilibrium or non-equilibrium conditions. In serine aminoacylation system it seems that the enzymes from different organisms are much more diverse than cognate tRNAsSer, which usually comprise rather conserved major recognition elements. In the present study we explored heterologous serylation among macromolecular partners from various organisms or cellular compartments. Different seryl-tRNA synthetases (SerRS) showed variable degree of flexibility in cross-domain charging, which is often unilateral. The most stringent is the recognition of cognate tRNA by Escherichia coli SerRS, somewhat more flexible is the counterpart from from Saccharomyces cerevisiae, followed by SerRS from methanogenic archaea Methanococcus jannaschii and Methanococcus maripaludis, which serylate tRNASer from all three domains of life. Interestingly, methanogenic SerRS from the third archaeon Methanopyrus kandleri discriminates yeast tRNASer. Maize organellar SerRS aminoacylates tRNAsSer of bacterial origin.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

POVEZANOST RADA