Naslov
Antitumor activity of non-aromatic naphthalane: angiogenesis inhibition in murine oral squamous cell carcinoma

Autori
Alajbeg, Ivan ; Ivanković, Siniša ; Jurin, Mislav ; Pirkić, Ahmed ; Alajbeg, Z. Iva ; Cekić-Arambašin, Ana ; Zečević, Željka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of 7th biennal congress of European Association of Oral Medicine / Reichart, Peter - Berlin : Quintessenz Verlags-GmbH, 2004, 38-38

Skup
7th biennal congress of European Association of Oral Medicine

Mjesto i datum
Berlin, Njemačka, 23.09.2004. - 25.09.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
naphthalane; oral squamous cell carcinoma; animal model; immunohistochemistry; angiogenesis

Sažetak
Special Croatian natural petrol fraction referred to as "non-aromatic very rich in steranes (NAVS) naphthalane" is derived from ordinary brown naphthalane, used for decades in the treatment of vulgar psoriasis, by removing the polycyclic aromatic content and by concentrating sterane content. Because the structure of NAVS steranes are similar to those of bioactive compounds, such as glucocorticoids and vitamin D, and due to encouraging previous in vitro and in vivo studies on its inhibitory effect on squamous cell carcinoma (SCC), we investigated the antiangiogenic effect as the basis of its antiproliferative activity. We have used a model in which 100 μ l suspension of 105 SCC VII cells were implanted in buccal pouch of 48 singeneic C3H mice via an intra-oral route. Seven days after inoculation buccal tumors were formed and animals were divided into 6 treatment groups. Each treatment group received intratumoral injection of one of the following: paraffin oil (PO) (as negative controls) ; NAVS (7th and 14th day, respectively) ; 1, 25-dihydroxyvitamin D3 (1, 25-D3) (as positive controls) ; combination of NAVS and 1, 25-D3 ; and combination of PO and 1, 25-D3. Tumor growth was assessed by weekly measurements. Animals were killed after 1, 2 and 3 weeks, and tumors were submitted for microscopic evaluation. Microvessel density counts were assessed by CD 34 immunohistochemistry staining analysis. Compared to PO group, tumor growth and angiogenesis were markedly decreased in 1, 25-D3 and NAVS groups, being lower in former group, and survival rate was highest in NAVS group. NAVS may decrease oral SCC growth by inhibiting vascular proliferation.

Izvorni jezik
Engleski

Znanstvena područja
Dentalna medicina

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