Naslov
Loss of heterozygosity testing using real-time PCR analysis of single nucleotide polymorphisms

Autori
Čačev, Tamara ; Jokić, Mladen ; Spaventi, Radan ; Pavelić, Krešimir ; Kapitanović, Sanja

Izvornik
Journal of cancer research and clinical oncology (0171-5216) 132 (2006), 3; 200-204

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
real-time PCR; SNP; E-cadherin

Sažetak
Colon cancer is a genetic disease, caused by mutations in different oncogenes and tumor-suppressor genes. The initial escape of a cancer cells from the primary tumor requires changes in cell-cell adhesion switch, which in epithelial tumors, is mediated mainly by members of the cadherin family. The aim of our study was to investigate the LOH at the E-cadherin gene locus in sporadic colon cancer as well as to evaluate the usefulness of real-time PCR SNP analysis as a new technique in this type of studies. 100 cases of human sporadic colon cancer and corresponding normal tissue samples were analyzed using 2 flanking polymorphic markers commonly used in the LOH analysis at the E-cadherin gene locus by conventional VNTR-LOH analysis. Two intragenic E-cadherin SNP markers were analyzed using real-time PCR SNP analysis. 17, 6 % of LOH was detected using flanking markers, however no LOH was detected when the intragenic E-cadherin SNP markers were introduced into our study. Since these markers are intragenic they more accurately represent the status of the E-cadherin gene than the previously used flanking markers. In conclusion, real-time PCR SNP analysis was found to be more accurate, faster, simpler and more high-throughput method than the conventional VNTR-LOH analysis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA