Naslov
Interfacial characteristics of marine algae and bacteria using adhesion based electrochemical sensor

Autori
Kovač, Solveg ; Ivošević, Nadica ; Svetličić, Vesna ; Žutić, Vera

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Limnology and Oceanography: Navigation into the Next Century, abstract book / The American Society of Limnology and Oceanography - Waco (TX) : The American Society of Limnology and Oceanography, 1999

Skup
Limnology and Oceanography: Navigation into the Next Century

Mjesto i datum
Santa Fe (NM), Sjedinjene Američke Države, 01.02.1999. - 05.02.1999

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
adhesion based electrochemical sensor; marine algae; marine bacteria

Sažetak
Mixtures of biopolymers and microbial cells are typical of natural aquatic environment such as seawater where they form biofilms at the interfaces and undergo aggregation. The electrochemical sensor that we have developed enables a distinction between cell adhesion and biopolymer adsorption. We study the adhesion of phytoplankton and bacteria in presence of adsorption of dissolved biopolymers at a model interface: seawater/dropping mercury electrode where the interfacial forces can be controlled by applied potential. Measuring parameters are frequency of cell adhesion, and the time of monolayer formation at the electrode surface. Upon impact of a D.tertiolecta cell with the interface a fast deformation occurs, followed by rupture of its fluid membrane and spreading of the cell content on the mercury surface. The electrical signal carries the information on all phases of the adhesion process starting from the initial molecular contact to the spreaded film. Marine bacteria adhere directly at the DME forming molecular contact, but because of rigid cell wall and the smaller size the adhesion signals of individual cells can not be resolved. We have demonstrated that the cell attachment and biopolymer adsorption occur simultaneously forming separated domaines in the initial film.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA