Naslov
Correlation between glutathione peroxidase activity and the quantity of selenium in the whole blood of beef calves

Autori
Harapin, Ivica ; Bauer, Mario ; Bedrica, Ljiljana ; Potočnjak, Dalibor

Izvornik
Acta veterinaria Brno (0001-7213) 69 (2000), 2; 87-92

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
glutathione-peroxidase; selenium; white muscle disease; beef cattle

Sažetak
The aim of the study was to establish concentration of selenium and the pertinent activity of glutathione peroxidase (GSH-Px) in whole blood that could be used as reference values in future research. The first part of study was carried out on clinical healthy beef calves (n = 35), fed a basal diet and a ready-made fodder mix that contained 0.1 mg.kg(-1) selenium. In the second part of our research, we investigated the GSH-Px activity in a group of calves (n = 47) that had not received supplements added to the basal diet. The research was carried out in the north-west Croatia, in the region known to be poorer in selenium. We determined GSH-Px activity in whole blood by the "Ransel" method adapted to a Technicon RA-1000 at 37 degrees C. Determination of Se concentration was done with a modification of the method given by Perkin-Elmer HGGS. The mean value of the Se concentration in the whole blood of the first group of calves was 200.22 +/- 45.2 mu g.l(-1) and pertinent average GSH-Px activity was 764.6 +/- 197.8 mu kat.l(-1). From the results obtained, a correlation between Se and GSH-Px was calculated (r = 0.82 ; P < 0.001). The mean values of the GSH-Px activity in herd that did not receive a supplement were 435.3 +/- 155.76 mu kat.l(-1). Out of 47 animals of the second herd, four animals (8.51%) had lower values than those recommended as sufficient. This study confirmed that after the calculation of the correlation between Se and GSH-Px, glutathione peroxidase activity determination can be used as a rapid and simple proxy for the determination of selenium concentration in whole blood.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA