Pregled bibliografske jedinice broj: 16210
Systemic excision of vector sequences from the BAC-cloned herpesvirus genome during virus reconstitution
Systemic excision of vector sequences from the BAC-cloned herpesvirus genome during virus reconstitution // Journal of virology, 73 (1999), 8; 7056-7060 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 16210 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Systemic excision of vector sequences from the BAC-cloned herpesvirus genome during virus reconstitution
Autori
Wagner, Markus ; Jonjić, Stipan ; Koszinowski, Ulrich H. ; Messerle, Martin
Izvornik
Journal of virology (0022-538X) 73
(1999), 8;
7056-7060
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
cytomegalvirus; mutagenesis; bacterial arteficial chromosome (BAC)
Sažetak
Recently the mouse cytomegalovirus (MCMV) genome was cloned as an infectious bacterial arteficial chromosome (BAC) (Messerle M., I. Crnković, W. Hammerschmidt, H. Ziegler, and U.H. KOszinowski. 1997. Proc. Natl. Acad. Sci. USA 94:14759-14763). The virus obtained from this construct is attenuated in vivo due to deletion of viral sequences and insertion of the BAC vector. We reconstituted the full-lenght MCMV genome and flanked the BAC vector with identical viral sequences. This new construct represents a versatile basis for construction of MCMV mutant ssince virus generated from the construct loses the bacterial sequences and acquires wildtype properties.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti
POVEZANOST RADA
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
