Naslov
Indukcija diferencijacije neuroblasta čimbenicima rasta u embrijima sisavaca u kulturi organa in vitro
(Growth factors induced neuroblasts differentiation in mammalian embryo in organ culture in vitro)

Autori
Crnek-Kunstelj, Vesna ; Stipić, Jagoda ; Bradamante, Želimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings of Abstracts of Eighth Croatian Biological Congress with International Participation / / Besendorfer, Višnja ; Kopjar, Nevenka - Zagreb : Hrvatsko biološko društvo, 2003, 84-85

Skup
8th CROATIAN BIOLOGICAL CONGRESS with International Participation

Mjesto i datum
Zagreb, Hrvatska, 27.09.2003. - 02.10.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Fibroblastni faktor rasta; neutrofični faktor rasta živaca; embrio štakora; kultura in vitro
(Fibroblast growth factor; Neurotrophic nerve growth factor; Rat embryo; In vitro culture)

Sažetak
Fibroblast growth factor (FGF) was investigated as a mesoderm inducer, also as neural inducer. Nerve growth factor (NGF) has a trophic role for the distinct neural population in peripheral nervous system. 14-days organ culture of postimplantation rat embryo was established to investigate the role of FGF and NGF in mammylian development. Embryos were treated 100 ng/ml and 200 ng/ml of FGF and NGF, and with both agents combined. NGF did not improve differentiation of neuroblasts. The differentiation of neuroblasts was significantly higher in FGF-treated embryos (more obviously when 200 ng/ml was used) and about the same as in NGF/FGF-treated embryos. Neuronal differentiation is probably initially regulated with FGF signal rather than NGF.

Izvorni jezik
Hrvatski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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