Pregled bibliografske jedinice broj: 161737
Sinergistički učinak čimbenika rasta FGF-a i NGF-a na regulaciju rasta kultiviranih zametaka sisavaca
Sinergistički učinak čimbenika rasta FGF-a i NGF-a na regulaciju rasta kultiviranih zametaka sisavaca // Proceedings of Abstracts of Eighth Croatian Biological Congress with International Participation / Besendorfer, Višnja ; Kopjar, Nevenka (ur.).
Zagreb: Hrvatsko biološko društvo, 2003. str. 82-84 (poster, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 161737 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Sinergistički učinak čimbenika rasta FGF-a i NGF-a na regulaciju rasta kultiviranih zametaka sisavaca
(Growth factors NGF and FGF act synergistically in regulation of growth in cultivated mammalian embryos)
Autori
Crnek-Kunstelj, Vesna ; Stipić, Jagoda
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Proceedings of Abstracts of Eighth Croatian Biological Congress with International Participation
/ Besendorfer, Višnja ; Kopjar, Nevenka - Zagreb : Hrvatsko biološko društvo, 2003, 82-84
Skup
8th CROATIAN BIOLOGICAL CONGRESS with International Participation
Mjesto i datum
Zagreb, Hrvatska, 27.09.2003. - 02.10.2003
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
Fibroblastni faktor rasta; neutrofični faktor rasta živaca; embrio štakora; kultura in vitro
(Fibroblast growth factor; Neurotrophic nerve growth factor; Rat embryo; In vitro culture)
Sažetak
FGF plays an important role in vivo in cell proliferation and differentiation in embryogenesis, tissue regeneration, CNS development, wound healing and tumor progression. NGF is significant for the survival and development of symphatetic and neural crest-derived sensory neurons. The growth of rat embryo in vitro in response to FGF and NGF signals and their possible synergistic action was investigated. Rat embryos in gastrulation phase were treated with 100 ng/ml and 200 ng/ml of FGF and NGF, and with both agents combined in serum-free (MEM)and serum supplemented medium. Growth of explants was evaluated by measuring average size of explants during 14 days of culture. Both concentrations of NGF impaired growth, while FGF stimulated it, even it was not always significantly different from control groups in a paticular measure-day. When NGF and FGF were applied together in serum-supplemented medium, the growth was significantly improved compared to control and other experimental groups. It seems that NGF and FGF may act synergistically, thus improving growth of embryos in serum-supplemented medium.
Izvorni jezik
Hrvatski
Znanstvena područja
Biologija, Temeljne medicinske znanosti
