Naslov
The Effect of Cadmium Chloride and Vitamin C on DNA Damage in V79 Cells

Autori
Kašuba, Vilena ; Rozgaj, Ružica ; Trošić, Ivančica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, pregledni

Izvornik
Final Program and Abstract Book, CROTOX 2004 3 rd Croatian Congress of Toxicology, Plitvice Lakes, Croatia, May 26-29, 2004. / - Zagreb, 2004

Skup
CROTOX 2004 3 rd Croatian Congress of Toxicology, Plitvice Lakes, Croatia, May 26-29, 2004

Mjesto i datum
NP Plitvička jezera, Hrvatska, 26.05.2004. - 29.05.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
cadmium chloride; Vitamin C; V79 cells; genotoxic effect

Sažetak
In the present work, the ability of Vitamin C to modulate genotoxic effects of cadmium chloride on Chinese hamster V79 cells was assessed using alkaline single cell gel electrophoresis (comet assay). The comet assay detects release of the DNA from a highly supercoiled DNA-protein complex. The extent of migration is proportional to the number of breaks in DNA. Tail length ana tail moment reflect DNA damage. Chinese hamster V79 cells were treated with various concentrations of cadmium chloride (10-4 M, 10-5 M, 10 -6 M and 10 – 7 M) for 24 hours, with, or without pre-treatment with 100 microM ascorbic acid three hours before cadmium chloride was added. Three separate cultures per concentration were analysed. The tail lengths and tail moments of 100 cells per culture were evaluated. To evaluate statistical significance of the data, one-way analysis of variance was used. In addition, a Tukey Post Hoc comparison of means for unequal N was performed to test statistical significance between groups. Cadmium chloride increased tail length and tail moment. The results showed significant difference between both supplemented or un-supplemented 10-4 M CdCl2 and all other groups, but there was not difference in the effect between these two groups. Protective role of Vitamin C was not confirmed at all tested concentrations of cadmium chloride. Our results showed that the alkaline comet assay might be considered as a good test for measuring early heavy metal DNA damage in Chinese hamster V79 cells.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Javno zdravstvo i zdravstvena zaštita

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