Naslov
Protein transduction as a tool for delivering of drugs into the cells

Autori
Grdiša, Mira

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
International Conference on the Interfaces among Mathematics, Chemistry & Computer Sciences / Graovac, Ante - Zagreb : Nakladnik, 2004

Skup
19th International Conference on the Interfaces among Mathematics, Chemistry & Computer Sciences

Mjesto i datum
Dubrovnik, Hrvatska, 22.06.2004. - 28.06.2004

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
TAT-protein; cell lines; cell cycle

Sažetak
Delivery of macromolecular drugs (e.g. antisense oligonucleotides, polymer-drug conjugates, etc.) designed to work in specific sites inside cells is complicated as macromolecules typically have access to fewer biological compartments than small molecules. To achieve an efficient intracellular drug delivery into the cells, TAT peptides derived from the HIV-1 TAT protein facilitate intracellular delivery of proteins and small colloidal particles. TAT fusion protein enters into the cells when added to the surrounding media, in concentration depended manner. A TAT component is degraded by proteolytic enzymes, protein is refolded with cell chaperons (Hsp proteins) and ready for physiological function. In general, plasma membranes of cells are impermeable to proteins and peptides. Because of that, the potential for intracellular therapeutic use of proteins, peptides and oligonucleotides has been limited by the impermeable nature of the cell membrane to these compounds. Thus protein transduction has been overcame that obstacle, and it has been widely used to analyze biochemical processes in living cells. The present study showed how transduced proteins influence on the regulation of cell cycle. The proteins (p27, p23, Mp27) were transdused into different cell lines (NALM, MOLT, Raji, SuDHL, and K562) and their effects on proliferation of the cells were measured. A transduced p27 did not remarkable influence on proliferation of examined cell lines. Mutated p27 inhibited the proliferation of examined cell lines up to 30 %. On the other hand, a transduction of p23 protein, truncated form of p27, inhibited the proliferation all of examined cell lines 30-60 %. Also the effects on expression of host p27 protein were examined, as well as an influence on induction of apoptosis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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