Naslov
Synthesis and Antitumor Activity of 5-Bromo-1-mesyluracil

Autori
Glavaš-Obrovac, Ljubica ; Karner, Ivan ; Pavlak, Marina ; Radačić, Marko ; Kašnar-Šamprec, Jelena ; Žinić, Biserka

Izvornik
Nucleosides, nucleotides & nucleic acids (1525-7770) 24 (2005), 5-7; 557-569

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
N-1-Sulfonylpyrimidine derivatives; Human cervix carcinoma cell (HeLa); Enzyme activity; Anaplastic mammary carcinoma

Sažetak
Large scale preparation of 5-bromo-1-mesyluracil (BMsU) 4 has been optimized. BMsU was synthesized by condensation of silylated 5-bromouracil and MsCl in acetonitrile or by the reaction of 5-bromouracil with MsCl in pyridine. The same product was obtained by bromination of 1-mesyluracil. The purpose of this study was to elucidate the effects of BMsU on the biosynthetic activity of tumor cell enzymes involved in DNA, RNA and protein syntheses, and in de novo and salvage pyrimidine and purine syntheses. Investigations were performed in vitro on human cervix carcinoma cells (HeLa). BMsU displayed inhibitory effects on DNA and RNA syntheses in HeLa cells after 24 hours of treatment. De novo biosynthesis of pyrimidine and purine was also affected. Antitumor activity of BMsU is closely associated with its inhibitory activity on the enzymes that play an important role in the metabolism of tumor cells. In vivo antitumor activity of BMsU was also investigated. The model used in investigations was a mouse anaplastic mammary carcinoma transplanted into the thigh of the right leg of CBA mice. Significant reduction in tumor growth time was achieved with BMsU administrated at a dose of 50 mg/kg.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

POVEZANOST RADA