Naslov
Murine spermatogonial stem cells: targeted transgene expression and purification in an active state

Autori
Giuili, Galicia ; Tomljenović, Andrea ; Labrecque, Natalie ; Oulad-Abdelghani, Mustapha ; Rassoulzadegan, Minoo ; Cuzin, Francois

Izvornik
EMBO reports (1469-221X) 3 (2002), 8; 753-759

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
stem cell; spermatogonia

Sažetak
A 400 bp fragment of the spermatogonia-specific Stra8 locus was sufficient to direct gene expression to the germinal stem cells in transgenic mice. A fractionation procedure was devised, based on immunomagnetic sorting of cells in which the promoter drives the expression of a surface functionally neutral protein tag. The purified cells expressed the known molecular markers of spermatogonia Rbm, cyclin A2 and EP-Cam, and the beta(1)- and alpha(6)-integrins characteristic of the stem cell fraction. A 700-fold enrichment in stem cells was determined by the ability of the purified fractions to re-establish spermatogenesis in germ cell-depleted recipient testes.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA