Naslov
Anodic Sampling of Standard Elements in Noble Dental Alloys (In, Sn, Pt, Zn, Cu) by Thin-Layer Chromatography

Autori
Jasprica, Ivona ; Živko-Babić, Jasenka ; Medić-Šarić, Marica ; Mornar, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
HPTLC International Symposium 2003 / - , 2003

Skup
HPTLC International Symposium 2003

Mjesto i datum
Lion-sur-Mer, Francuska, 15.10.2003. - 18.10.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Anodic sampling; Dental alloys; TLC

Sažetak
Different dental alloys are used in dentistry as crowns, bridges, combined restorations, dental implants and orthodontic wires. In theory, up to 14 different alloys can be incorporated in one oral cavity. To avoid the problem of polymetalism or alergic reactions it is necessary to know the composition of an alloy. In our work we used anodic sampling method combined with thin-layer chromatography to establish the behaviour of indium, tin, platinum, zinc and copper on thin layer. The analysis was performed in water-HCl (conc.) solution in a 10:1 volume ratio using a 4.5 V battery. The time of sampling was 30 seconds and transfer of dissoluted cations to the chromatographic plates took 15 seconds. Standard solutions (In2(SO4)3, SnCl2, Cl6H2Pt, ZnSO4, CuCl2 0.1 mg/ml) and samples of standard elements were applied to HPTLC plates 10x10 cm, precoated with cellulose. Plates were developed in saturated glass chamber to the distance of approximately 8 cm. Mobile phase was iso-amyl alchohol (3-methyl-1-butanol) – HCl (36.5 %) – acetonitrile in a volume ratio 5.4:4.3:0.3. After development, plates were dried in a steam of hot air and the spots were visualised by spraying with the saturated ethanolic solutions of alizarin, diphenylcarbazide and quercetin. The plates were dried and exposed to NH3 vapour. We found that described method is suitable for the analysis of indium, tin, platinum, zinc and copper. It is important to have those preliminary results so dental alloys of unknown composition could be analysed.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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