Naslov
Influence of orally-administered probiotic strain Lactobacillus acidophilus M92 on the gastrointestinal microflora of mice

Autori
Frece, Jadranka ; Kos, Blaženka ; Beganović, Jasna ; Vuković, Snježana ; Šušković, Jagoda

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Treći hrvatski mikrobiološki kongres s međunarodnim sudjelovanjem

Mjesto i datum
Poreč, Hrvatska, 04.07.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
adhesion; intestinal microflora; Lactobacillus acidophilus; probiotic properties

Sažetak
Adhesion to intestinal epithelial cells is an important prerequisite for colonization of probiotic strains in the gastrointestinal tract, preventing their immediate elimination by peristalsis and providing a competitive advantage in this ecosystem. The aim of this work was to investigate adhesiveness of Lactobacillus acidophilus M92 to mouse ileal epithelial cells in vitro. Subsequently, adhesiveness and survival in vivo was monitored by measuring the faecal and intestinal level of L. acidophilus M92 after oral administration of this strain on mice. During the feeding of mice with L. acidophilus M92 with daily dose of 2.2x10on10 cells, the number of lactobacilli in faeces was increased and reduction of enterobacteria and sulphite-reducing clostridia was observed. The similar results were obtained in homogenates of small and large intestine of mice in the 1st and 14th day after feeding with L. acidophilus M92, which pointed out adherence and colonisation ability of examined strain. These results confirmed adhesiveness ability of L. acidophilus M92 to mouse ileal epithelial cells obtained in vitro. This investigation has shown that L. acidophilus M92 has ability to establish in the human gastrointestinal tract alters the composition of the intestinal microflora and faeces, what is an important determinant in the choice of probiotic strains.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA