Naslov
Immunomodulatory effect of Lactobacillus rhamnosus GG from low fat fresh cheese "BioAktiv LGG"

Autori
Mahnet, Stjepan ; Kirin, Slavko ; Kos, Blaženka ; Frece, Jadranka ; Šušković, Jagoda

Izvornik
Mljekarstvo (0026-704X) 54 (2004), 1; 5-18

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Lactobacillus rhamnosus; probiotic properties; adhesion; immunomodulation; antibodies; ELISA

Sažetak
Various immune responses have been influenced by probiotics and these immunomodulatory effects have been proposed for several potential applications such as prevention of infectious diarrhoea, allevation of hypersensitivity reactions and tumor suppression. The new probiotic product from LURA d.d., low fat fresh cheese "BioAktiv LGG", contains well-known probiotic strain Lactobacillus rhamnosus GG (LGG). The viable count of L. rhamnosus GG remained stable and has beeen 1.3x10 on 8 CFU/g of low fat fresh cheese "BioAktiv LGG" during storage of 16 days at 4oC. The effect of L. rhamnosus GG from this product on the immune response, as an important point in relation to immunomodulation, and its survival/residence time in the gastrointestinal tract in Swiss albino mice was also studied. During the feeding of mice with L. rhamnosus GG, with daily dose of 2.2 x10 on 10 cells, the number of lactobacilli in faeces was increased and reduction of enterobacteria and sulphite-reducing clostridia was observed. The similar results were obtained in homogenates of large intestine of mice in the 1st and in the 14th day after feeding with L. rhamnosus GG, which pointed out the adherence ability of examined strain. Furthermore, the oral immunization of Swiss albino mice was performed with daily dose of 2.2 x10 on 10 L. rhamnosus GG cells per day, for eight consecutive days. The blood samples were collected 4th, 8th, 10th, 14th and 21st day after 1st immunization. The sera of mice were tesed for total and specific anti-lactobacilli IgA, IgG and IgM antibodies by ELISA assay. L. rhamnosus GG slightly stimulated the total humoral immune response but did not evoke reaction against itself, which confirms its probiotic properties and make new probiotic product, low fat fresh cheese "BioAktiv LGG", as good carrier for this probiotic strain.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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