Naslov
Use of commercial ELISA kit for Salmonella typhimurium antibodies in commercial chicken layers during a field outbreak

Autori
Savić, Vladimir ; Šimpraga, Borka ; Balenović, Mirta ; Krstulović, Fani ; Lukač Novak, Irena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
Zbornik = Proceedings / Balenović, Mirta ; Wittner, Velimir - Zagreb : Hrvatsko mikrobiološko društvo, 2004, 65-65

Skup
Treći hrvatski mikrobiološki kongres s međunarodnim sudjelovanjem

Mjesto i datum
Poreč, Hrvatska, 04.10.2004. - 07.10.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Salmonella enterica Typhimurium; chicken layers; ELISA

Sažetak
Salmonella enterica Typhimurium, beside S. enterica Enteritidis, presents major threat for poultry meat and egg consumers worldwide, thus many countries are trying to control salmonella infections in poultry by compulsory testing of commercial poultry. Isolation of these pathogens from poultry and eggs undoubtedly gives the best insight into infection, but false negative results are commonly obtained from inapparent carriers. For that reason, many regulatory diagnostic programs for the detection of Salmonella infections in commercial poultry flocks rely on rapid Pullorum agglutination tests to screen birds because of the shared antigens of S. enterica Pullorum and other D group salmonellae including S. enterica Enteritidis. Since S. enterica Typhimurium, as a B group salmonella, can be easily omitted by this test, several commercial ELISA tests have been developed for S. enterica Typhimurium antibodies in chickens. These ELISA tests were evaluated mainly in birds after experimental S. enterica Typhimurium infection, whereas data from field outbreaks are few. We have evaluated the Guildhay commercial ELISA for detection of S. enterica Typhimurium antibodies during a spontaneous S. enterica Typhimurium outbreak in commercial chicken layers. Due to health problems, increased mortality and slight drop in egg production in 57-week-old flock of 700 chicken layers, S. enterica Typhimurium was isolated from liver, heart and ovary of two carcasses. Culturing of the spleens gave negative result. The birds were then treated with enrofloxacin for seven days and afterwards no mortality was recorded in the flock. Culturing of random samples of 30 eggs in age of 59 weeks as well as 61 weeks gave negative result. Sixty serum samples were tested for presence of S. enterica Typhimurium antibodies at age of 57 weeks, 59 weeks as well as at age of 61 weeks. No suspect or positive serum was found at age of 57 weeks i.e. when S. enterica Typhimurium was isolated from the carcasses, whereas 8 (13.3%) suspect and 2 (3.3%) positive samples were found two weeks later and 5 (8.3%) suspect and 2 (3.3%) positive samples were found four weeks after the outbreak. It can be concluded that the commercial ELISA kit is a useful tool for detecting S. enterica Typhimurium positive chicken flocks, particularly where isolation of the microorganism can be impeded for any reason, including use of antibiotics. It seems that the way of sampling and sample size may play very important role, particularly in chickens kept in cage systems where horizontal spread can be limited.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

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