Naslov
Differentiation of infectious bursal disease virus strains by single strand conformational polymorphism analysis

Autori
Lojkić, Ivana ; Biđin, Zdenko ; Husnjak, Koraljka ; Pokrić, Biserka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Zbornik = Proceedings / Treći hrvatski mikrobiološki kongres s međunarodnim sudjelovanjem, Poreč, 4.-7. listopada 2004. / Balenović, Mirta ; Wittner, Velimir - Zagreb : Hrvatsko mikrobiološko društvo, 2004, 100-100

Skup
Treći hrvatski mikrobiološki kongres s međunarodnim sudjelovanjem

Mjesto i datum
Poreč, Hrvatska, 04.10.2004. - 07.10.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
infectious birsal disease virus; field isolates; single-stranded DNA; electrophoresis

Sažetak
Single-strand conformational polymorphism (SSCP) analysis of polymerase chain reaction (PCR) amplified products is a sensitive technique for rapid scanning of mutations occurring in single stranded DNAs. It is based on the fact that single stranded DNAs differing by a single base substitution may form different conformers. The conformational changes can be detected as the alterations in the electrophoretic mobility in non-denaturating polyacrylamide gels. The SSCP method was applied by us for differentiation of infectious bursal disease virus (IBDV) isolates. Field isolates were analyzed for variations in 422-bp fragment of VP2 gene. The single stranded DNAs, obtained by treatment of PCR products by alkali denaturing buffer (ADB), were electrophoretically analyzed in 8 % polyacrilamide gel. The SSCP analysis showed the difference between analyzed IBDV field isolates and confirmed our previous results of isolate differentiation carried out by restriction analysis using Cfo I, Sac I, Ssp I, Stu I and Taq I enzymes (Lojkić et al. (2003) Avian Dis. 47: 59).

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Veterinarska medicina

POVEZANOST RADA