Naslov
Influence of 864 MHz electromagnetic field on growth kinetics of established cell line

Autori
Pavičić, Ivan ; Trošić, Ivančica

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
46th International Symposium Electronics in Marine (Elmar-2004) : proceedings / Kos, Tomislav ; Grgić, Mislav - Zadar : Zagreb : Croatian Society Electronics in Marine ; Faculty of Electrical Engineering and Computing, 2004, 396-401

Skup
International Symposium Electronics in Marine (46 ; 2004)

Mjesto i datum
Zadar, Hrvatska, 16.06.2004. - 18.06.2004

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
864 MHz microwave ; TEM cell ; V79 cell culture ; growth kinetics

Sažetak
Considering often contradictory data on biological effects of mobile phones frequencies on established cell culture lines, our study aimed at evaluating the influence of 864 MHz electromagnetic field on proliferation, colony forming ability and viability of Chinese hamster lung cells of line V79 cell. Prior to exposure for 1, 2 and 3 hours in transversal electromagnetic mode cell (TEM-cell) cell culture were sub-cultivated for one day. Cells were exposed to 864 MHz continuous wave (CW) at an average specific absorption rate (SAR) of 0.66 W/kg. Philips PM 5508 connected with signal amplifier was used as signal generator. To determine cell growth, V79 cells were plated in concentration of 1x104cells/ml, and raised in a humified atmosphere at 37 °C in 5% CO2. Cell proliferation was determined by cell counts for each hour of exposure on post-exposure days 1, 2, 3, 4 and 5. To identify colony-forming ability, cells were cultivated in concentration of 40 cells/ml, and incubated as above. Colony forming ability for each hour of exposure was defined by colony counts on experimental day 7. Trypan blue exclusion method was used to determine cell viability. In comparison to sham-exposed cells, growth curve of irradiated culture samples showed significant decrease (p<0.05) after 2 and 3 hours of exposure on experimental day 3, respectively. Both, the colony forming ability and viability of irradiated cells did not significantly differ from sham-exposed controls. In conclusion, no vigorous influence of 864 MHz electromagnetic field on growth kinetics of V79 cell culture was detected. Where effect was seen, it was not dose-dependent.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

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