Naslov
Micronuclei and nuclear buds in genotoxicity assessment of the pesticide formulation Deherban A&reg ;

Autori
Želježić, Davor ; Garaj-Vrhovac, Vera

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedins of the 3rd Croatian Congress of Toxicology with International Participation / - Zagreb, 2004, 93-93

Skup
3rd Croatian Congress of Toxicology

Mjesto i datum
Plitvička Jezera, Hrvatska, 2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
herbicides; lymphocytes; micronucleus
(herbicidi; limfociti; mikronukleus)

Sažetak
Pesticides of worldwide application are used in agriculture in vast amounts each year, of which herbicides are the most prominent class. Phenoxy herbicides constitute one of the largest groups of herbicides sold in the world. Among them, for many years 2, 4-dichlorophenoxyacetic acid has been the one most used. In the present study micronucleus assay have been used to assess possible the chromosome-damaging activity of the pesticide formulation Deherban A&reg ; containing 500 mg/ml of 2, 4-dichlorophenoxyacetic acid as an active ingredient. Human peripheral blood lymphocytes were treated with the pesticide for 24 hours in the absence and in the presence of the rat liver microsomes as metabolic activation system. Two different concentrations of pesticide expressed as the concentration of the active ingedient 2, 4-D have been tested (0, 4 mg/ml and 4 mg/ml). In addition to micronucleus assay, the frequency of nuclear buds in binucleated lymphocytes was analyzed. The total number of micronuclei and nuclear buds was significantly increased in the treated lymphocytes compared to the control for both concentrations tested. The number of binucleated lymphocytes with more than 1 micronucleus was higher in treated lymphocytes compared to the control but that difference was not statistically significant. The presence of the metabolic activator caused a significant increase in the number of micronuclei compared to the treatment in the absence of the S9 mix for both concentrations tested. The number of nuclear buds was significantly elevated by the presence of the metabolic activator only at the higher concentration of Deherban A&reg ; . The results of all studies concerning pesticide genotoxicity would be much more similar to the field exposure conditions if pesticide formulations were used instead of active ingredient, as it was the case in the paper presented.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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