Naslov
IMPROVED DETECTION OF RESIDUAL CELLULAR DNA BY USING SHORT MONOLITHIC COLUMNS

Autori
Forčić, Dubravko ; Branović, Karmen ; Ivančić, Jelena ; Barut, Miloš ; Štrancar, Aleš ; Jug, Renata ; Mažuran, Renata

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
First Summer School on Monoliths for Biochromatography, Bioconversion and Solid Phase Synthesis / Štrancar, Aleš - Ljubljana : BIA Separation, 2004, 61-61

Skup
First Summer School on Monoliths for Biochromatography, Bioconversion and Solid Phase Synthesis

Mjesto i datum
Portorož, Slovenija, 06.06.2004. - 09.06.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
residual cellular DNA; monilith
(residual cellular DNA; monolith)

Sažetak
Current knowledge suggests that a continuous-cell-line DNA can be considered as a cellular contaminant, rather than as a significant risk factor requiring removal to extremely low levels. This is based on the following: (a) the contaminating DNA in a biological product generally occurs as small fragments unlikely to encode a functional gene ; (b) the possible inactivation of any biological activity of contaminating DNA during the processing ; and (c) any reduction in the level of contaminating DNA during the purification process. On the basis of this reassessment it was concluded that levels up to 10 ng per purified dose can now be considered as acceptable. The purification process has to be validated by appropriate methods, including spiking studies, to demonstrate its capability to remove DNA to an acceptable level. Anion-exchange chromatography is one of the most important methods in the downstream processing of different biological macromolecules, both as a process and an analytical technique. This study shows that the eukaryotic genomic DNA can be successfully bound to the CIM&reg ; ; DEAE monolithic column from different biological solutions. We have developed a simple and fast chromatographic procedure for the isolation, purification and detection of genomic DNA that does not include the usage of toxic organic solutions.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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