Naslov
FAST PURIFICATION OF PLASMID DNA BY USING SHORT MONOLITHIC COLUMNS

Autori
Branović, Karmen ; Forčić, Dubravko ; Ivančić, Jelena ; Štrancar, Aleš ; Barut, Miloš ; Košutić Gulija, Tanja ; Zgorelec, Renata ; Mažuran, Renata

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
First Summer School on Monoliths for Biochromatography, Bioconversion and Solid Phase Synthesis / Štrancar, Aleš - Ljubljana : BIA Separation, 2004, 60-60

Skup
First Summer School on Monoliths for Biochromatography, Bioconversion and Solid Phase Synthesis

Mjesto i datum
Portorož, Slovenija, 06.06.2004. - 09.06.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
plazmid DNA; monilith
(plasmid DNA; monolith)

Sažetak
Anion-exchange chromatography is one of the most important methods in downstream processing of plasmid DNA, both as a process and an analytical technique. Separation of plasmid DNA on traditional particle based anion-exchange supports is usually slow. Moreover, such supports have a low capacity for plasmid DNA due to the steric exclusion effects. In this work, the separation of plasmid DNA using short monolithic columns, Convective Interaction Media, will be presented. It will be demonstrated that plasmid DNA can be purified from bacterial cells using alkaline lysis followed by chromatography on a very short weak anion-exchange chromatographic columns - disks - with good purity and quality within a short time. Furthermore, the separation of plasmid DNA from cell RNA can be carried out without the need of adding RNA-se. Fast and efficient method for in-process control of the purified plasmid will be described.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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