Naslov
Propagation of Sendai virus on MRC-5 cells

Autori
Cebalo, Karin ; Cvetko, Lidija ; Markotić, Alemka ; Gagro, Alenka ; Lončar, Ljubica ; Sladoljev, Srećko ; Rabatić, Sabina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Izvornik
Animal Cell Technology meets Genomics / Godia, Francesc - Granada, 2003

Skup
18th ESACT MEETING

Mjesto i datum
Granada, Španjolska, 11.05.2003. - 14.05.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Sendai virus; MRC-5

Sažetak
Sendai virus (SeV) belongs to the family Paramyxoviridae and structurally is an enveloped, single stranded negative sense RNA virus. It is highly cytopathic virus in different cell cultures, where it can induce apoptosis. Previous studies showed that human foetal lung fibroblasts (MRC-5) are suitable host cells for numerous viruses. We wanted to investigate whether SeV (usually propagated in the allantoic cavity of the chicken embryo) can grow on MRC-5 cells also. The virus titre was determined by the hemaglutination test. The first passage of SeV on MRC-5 cells gave the highest titre after two days and started to decline after day four, while in the second SeV passage, no virus titre was found. SeV induced cytopathic effect in MRC-5 cells. Previously it was found that SeV induced FAS (CD95)-independent apoptosis pathway in CV-1 cells. CD95 is a surface molecule that mediates apoptotic death on infected cells. We measured the expression of CD95 by flow cytometry and our preliminary results showed downregulation of CD95 expression on MRC-5 cells infected with SeV (propagated on chicken embryo). To investigate the possible role of cytokines/chemokines in the interaction of SeV with MRC-5 cells, we determined their levels in supernatants of infected cells by ELISA. Tumour necrosis factor alpha (TNF-alpha), interferon alpha (IFN-alpha), granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-6, IL-10, IL-12p70, IL-8, and macrophage chemoattractant protein-1 (MCP-1) were measured. In the first passage, SeV induced the production of MCP-1, GM-CSF and IL-6, while in the second passage only low IL-6 level was detected. Further studies are necessary to shed more light on the mechanisms involved in the interaction between SeV and MRC-5 cells.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA