Naslov
Genetic analysis of Puumala virus in newly discovered endemic region during great 2002 HFRS outbreak in Croatia

Autori
Cvetko, Lidija ; Markotić, Alemka ; Plyusnina, Angelina ; Turk, Nenad ; Margaletić, Josip ; Milas, Zoran ; Miletić-Medved, Marica ; Avšič-Županc, Tatjana ; Plyusnin, Alexander

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Conference Program & Abstracts / - , 2004, 139-139

Skup
The 6th International Conference on Hemorrhagic Fever with Renal Syndrome (HFRS), Hantavirus Pulmonary Syndrome (HPS) and Hantaviruses

Mjesto i datum
Seoul, Republika Koreja, 23.06.2004. - 25.06.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
HVBS; hantavirusi; Puumala; genetska analiza
(HFRS; hantaviruses; Puumala; genetic analysis)

Sažetak
Puumala virus (PUUV), causative agent of the mild type of hemorrhagic fever with renal syndrome (HFRS) belongs to the Hantavirus genus in the Bunyaviridae family. PUUV was shown to be dominant pathogen during the great HFRS outbreak in Croatia in year 2002. We focused our research on two newly discovered endemic areas (Okucani and Nova Gradiska) with the high number of reported HFRS cases and a significant increase of rodents population. Genetic analysis of wild-type (wt) Puumala virus strains from Croatia was performed. Strains were gained from its natural host reservoirs Clethrionomys glareolus originating from PUU-associated HFRS areas. Fifty seven bank voles were screened for the presence of PUU N antigen and 15 (26 %) were found to be positive. Total RNA isolated from rodent lung tissues was reverse transcribed followed by PCR amplification with medium (M) segment gene-specific primers. Partial sequences approximately 450 bp long of the wt-PUU M genome segment were recovered from the five C. glareolus. Phylogenetic analysis of the Croatian wt-PUU strains from the two localities revealed close relatedness of all five strains but without visible geographical clustering of genetic variants. On phylogenetic tree Croatian PUU strains clustered together with the strains from Austria. This confirms the idea that Croatian PUU falls into south-european genetic lineage, as was expected. Along with HFRS, another zoonosis - leptospirosis is very common at the same regions. Both etiologic agents are rodent-borne and are maintained in persistently infected small rodents. They share same rodent species as host reservoirs. It is known that one rodent species can carry more different pathogens. We confirmed existence of PUUV (by PCR) and a strain of Leptospira spp (isolation) in the same C. glareolus.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA