Naslov
Rapid ecologically acceptable method for wheat protein content determination-comparison of methods

Autori
Šebečić, Blaženka ; Balenović, Jadranka

Izvornik
Deutsche Lebensmittel-Rundschau (0012-0413) 6 (2001); 221-224

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
wheat protein content determination; Dumas' method; Kjeldahl method; comparison of methods

Sažetak
To display features of the rapid and simple automatic Dumas method for nitrogen determination in wheat flour the method was compared with the standard Kjeldahl method as a refential one. The method is based on full incineration of sample and on total nitrogen determination in relation to the thermal conductivity of the nitrogen-helium mixture. Method testing proceeded by comparing the level of nitrogen in wheat flour samples determined by both methods. Data analysis of the protein content of the samples as determined by these two methods showed a significant positive correlation (r=0.975). An assessment of the two methods' precision revealed that standard errors of the averages of parallel determinations were not essentially different, i.e., the results yielded by Dumas' method and standard Kjeldahl's methods were the same. This rapid, simple and readily performed method permits doing many tests daily, using up a small amount of the sample (0.1-1 g) and dispensing with the use of noxious chemicals. It can thus be recomended as a very simple method to use and constituting an ecologically acceptable replacement for the standard Kjeldahl's method.

Izvorni jezik
Engleski

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