Naslov
Endotoxin level measurement in the house dust by the end-point Limulus amoebocyte lysate method

Autori
Varnai, Veda Marija ; Macan, Jelena ; Plavec, Davor ; Jureša, Dijana

Izvornik
Arhiv za higijenu rada i toksikologiju (0004-1254) 55 (2004), 2-3; 175-181

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, stručni

Ključne riječi
endotoxin; lipopolysaccharide; Limulus polyphemus; LAL assay; house dust

Sažetak
Endotoxin is a lipopolysaccharide, a part of Gram-negative bacteria cell membrane, commonly present in general and many occupational environments. In this article method of sample preparation and endotoxin measurement in 16 samples of house dust from urban homes (Zagreb, Croatia) by end-point chromogenic Limulus amebocyte lysate (LAL) bioassay is described. The house dust was collected in cellulose filters by vacuuming the bedroom and living room floors, and kept frozen until assayed. Samples were extracted from filters with 0.05% solution of Tween-20 in endotoxin-free water. Serial dilutions of samples were measured in duplicates. Satisfying linearity of the standard curve (r = 0.983), adequate recovery (92 and 110%) and repeatability (coefficient of variation from 0 to 8.5%) were obtained. The endotoxin levels found in the house dust samples ranged from 4.8 to 200 EU/mg, with arithmetic mean of 49.5 EU/mg (standard error of the mean of 12.1 EU/mg), and were in the range of house dust endotoxin values obtained by other authors.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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