ࡱ> 5@<bjbj22 "XX9HHH\ \1 (AAA$Y R !HA=AAA;A8HA<0Hd pWc_:T 01 \  d\\ Hd,AAAAAAA\\d\\Preparing tagged and carrier-linked indole-3-acetic acid (auxin) by adding an aminoethyl group on the indole ring N. Ili1,2, I. Habua1, L. S. Barkawi3, S. Park3, J. D. Cohen3, and V. Magnus1 1Ruer Boakovi Institute, P.O. Box 180, HR-10002 Zagreb, Croatia; 2present address: Phytomedics Inc., Dayton, NJ 08810, USA; 3Department of Horticultural Science, University of Minnesota, St. Paul, MN 55108, USA Indole-3-acetic acid is a universally distributed biomolecule which functions as an important hormone (auxin) in plants and, in humans and mammals, has been implicated in the etiology of liver and kidney diseases, but has also been proposed as a cancer therapeutic. Improved analytical methods are needed, for isolating and quantifying both the compound itself and the response-mediating proteins, such as enzymes, receptors, and transcription factors. Here we introduce 5- and 6-(2-aminoethylindole)-3-acetic acids (1 and 2) which can, under mild conditions, be linked via their amino groups to carrier proteins and to a variety of tags. The compounds were synthesized by standard preparative methods. To demonstrate their useful properties they were coupled to bovine serum albumin and to biotin, including appropriate spacer modules. One of the protein conjugates was used to elicit the formation of monoclonal antibodies. These were found to recognize biotin conjugates of 2 at the nanomolar level, as shown by an enzyme-linked immunosorbent assay (ELISA) using streptavidin-coupled alkaline phosphatase/p-nitrophenol phosphate for detection.  EMBED ChemDraw.Document.6.0  :  , . @ B \ ^ j | ~   > ? B B F ӱۊqkk h-CJhTh-5CJmH sH hBV55CJmH sH h-5CJmH sH  h-5H*h-H*mH sH h-mH sH h- h-H*h->*H*mHsHh->*mHsHh-H*mHsHh-mHsH h-5CJhTh-5;B*CJphhjv5;B*CJph) 89:;<$da$d$da$ 9; ij4579;<h-j(C h-5CJUVjh-5CJUh-56CJmH sH h-5CJmH sH  h-CJ h-5CJh-56CJ &P . 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