Naslov
Preparing tagged and carrier-linked indole-3-acetic acid (auxin) by adding an aminoethyl group on the indole ring

Autori
Ilić, Nebojša ; Habuš, Ivan ; Barkawi, Lana S. ; Park, Seijin ; Cohen, Jerry D. ; Magnus, Volker

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts, 2nd Scientific Symposium with International Participation: 45 Years of Molecular Biology in Croatia, 50 Years of Double Helix (ISBN 953-6256-40-1) / Ambriović Ristov, Andreja ; Brozović, Anamaria - Zagreb : Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 2003, 26-26

Skup
2nd Scientific Symposium with International Participation: 45 Years of Molecular Biology in Croatia, 50 Years of Double Helix

Mjesto i datum
Zagreb, Hrvatska, 20.11.2003. - 21.11.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
auxin; carrier-linked and tagged indole-3-acetic acid; monoclonal antibody; ELISA; 5-(2-aminoethylindole)-3-acetic acid; 6-(2-aminoethylindole)-3-acetic acid

Sažetak
Indole-3-acetic acid is a universally distributed biomolecule which functions as an important hormone (auxin) in plants and, in humans and mammals, has been implicated in the etiology of liver and kidney diseases, but has also been proposed as a cancer therapeutic. Improved analytical methods are needed, for isolating and quantifying both the compound itself and the response-mediating proteins, such as enzymes, receptors, and transcription factors. Here we introduce 5- and 6-(2-aminoethylindole)-3-acetic acids which can, under mild conditions, be linked via their amino groups to carrier proteins and to a variety of tags. The compounds were synthesized by standard preparative methods. To demonstrate their useful properties they were coupled to bovine serum albumin and to biotin, including appropriate spacer modules. One of the protein conjugates was used to elicit the formation of monoclonal antibodies. These were found to recognize biotin conjugates of 6-(2-aminoethylindole)-3-acetic acid at the nanomolar level, as shown by an enzyme-linked immunosorbent assay (ELISA) using streptavidin-coupled alkaline phosphatase/p-nitrophenol phosphate for detection.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

POVEZANOST RADA