Naslov
An improvement in segregation of human butyrylcholinesterase phenotypes having the fluoride-resistant variants

Autori
Kovarik, Zrinka ; Simeon-Rudolf, Vera

Izvornik
Arhiv za higijenu rada i toksikologiju (0004-1254) 54 (2003), 4; 239-244

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Ro 02-0683; Carbamoylation; Fluoride-resistant variants; Human butyrylcholinesterase; Inhibition

Sažetak
Correct recognition of butyrylcholinesterase (BChE ; EC 3.1.1.8) variants in human serum is essential if patients susceptible to a prolonged reaction following treatment with short acting muscle relaxants, like suxamethonium, are to be reliably identified. The dimethylcarbamate Ro 02-0683 is used in standard procedures for BChE phenotyping by measuring residual activity after two hours of inhibition. Such a long inhibition time distinguishes well between the usual (U) and atypical (A), but less successfully the fluoride-resistant (F) variant. In this paper rate inhibition constants were determined from the time course of Ro 02-0683 inhibition of homozygous (FF) and heterozygous (UF and AF) BChE phenotypes ; 1.6x106, 2.7x106 and 6.2x106 mol-1min-1dm3 for AF, FF and UF, respectively. After only 30 min of inhibition the resolution between the phenotypes was even better than after two hours. Hence, determination of the residual activity after 30 min inhibition is recommended for the segregation of the suxamethonium sensitive fluoride-resistant variants.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA